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How to use peptides

Three-step protocol — reconstitute, draw, inject. Plus timing per peptide class, the six most common mistakes, and the kit list you can sourcing from any PH pharmacy.
DocPH-BEG-003
SeriesBG-001 / 03
Filed2026.04
StatusActive

Steps

03

Total Time

< 60 sec

Needle Gauge

31G

Storage

2–8°C

§ 01

What you will need before opening a vial

Required kit

Five items. All available at PH pharmacies and supply outlets — no prescription required for the consumables.

Peptide vial
Lyophilized (freeze-dried) powder
Bacteriostatic water (BAC water)
NOT regular water — BAC water contains benzyl alcohol to preserve the peptide
Insulin syringes
0.3 ml or 0.5 ml, 31G needle — available at PH pharmacies
Alcohol swabs
For sterilising vial tops and the injection site
Sharps container
Or a thick plastic bottle with a screw lid
§ 02

Step 1 of 3 · ~ 30 seconds

Reconstitute the peptide

Reconstitution converts the lyophilised powder into the injectable solution. Slow water addition and gentle swirling are the entire technique.

  1. R-01Clean both rubber tops (peptide vial and BAC water vial) with an alcohol swab
  2. R-02Draw 1–2 ml of BAC water into an insulin syringe
  3. R-03Slowly drip the water down the inside wall of the peptide vial — never spray onto powder
  4. R-04Gently swirl the vial for 1–2 minutes until the powder fully dissolves
  5. R-05Never shake vigorously — this denatures the peptide
  6. R-06Label the vial with the date of reconstitution
  7. R-07Store in the refrigerator (2–8°C) — good for 4–6 weeks
§ 03

Step 2 of 3 · concentration → volume

Calculate & draw your dose

The arithmetic is the same for every peptide: total mg in the vial divided by ml of BAC water added equals concentration. Then dose ÷ concentration equals draw volume.

Worked example

10 mg vial + 2 ml BAC water = 5 mg / ml

For a 500 mcg dose: draw 0.1 ml (10 units) on the insulin syringe

Use our retatrutide reconstitution calculator to plug in your vial size, BAC water volume, and target dose — it returns the exact insulin-syringe units to draw.

§ 04

Step 3 of 3 · ~ 10 seconds

Inject subcutaneously

Subcutaneous means into the fat layer just beneath the skin — not into muscle. Rotate sites daily to avoid lumps and scarring.

Best injection sites · rotate daily

  • Belly fat (2 inches from navel)
  • Love handles
  • Front or outer thigh
  • Back of upper arm

Injection technique

  1. I-01Clean the skin with an alcohol swab and let it dry
  2. I-02Pinch 2–3 inches of fat between your fingers
  3. I-03Insert the needle at a 90° angle (straight in)
  4. I-04Push the plunger slowly over 3–5 seconds
  5. I-05Remove the needle and do not rub the site
  6. I-06Dispose of the syringe in a sharps container immediately
§ 05

Frequency × time-of-day

Timing by class

Frequency varies by peptide. The GLP-1 class is once weekly because of its long half-life; healing and growth-hormone peptides are typically daily.

PeptideFrequencyBest time
Retatrutide / Tirzepatide / SemaglutideWeeklySame day each week
BPC-157DailyMorning or night
TB-5002× weeklyAny time
GHK-CuDailyMorning or night
CJC-1295 / IpamorelinDailyBefore bed (fasted)
§ 06

Six things that will ruin a vial

Common mistakes

Most failures are not exotic — they are one of these six. Read the list once and you will avoid every one.

  • Using regular sterile water instead of bacteriostatic water (degrades faster)
  • Shaking the vial vigorously (denatures the peptide)
  • Injecting into muscle instead of subcutaneous fat
  • Not rotating injection sites (causes lumps and scarring)
  • Storing reconstituted peptides at room temperature
  • Drawing air bubbles into the syringe
§ 07

Continue the induction

Next steps

Educational reference only. Not medical advice. Confirm peptide-specific dose, frequency, and storage with the manufacturer COA and a licensed clinician before any protocol.