How to use peptides

Five items. All available at PH pharmacies and supply outlets, with no prescription required for the consumables.

Peptide vial

Lyophilized (freeze-dried) powder

Bacteriostatic water (BAC water)

NOT regular water: BAC water contains benzyl alcohol to preserve the peptide

Insulin syringes

0.3 ml or 0.5 ml, 31G needle, available at PH pharmacies

Alcohol swabs

For sterilising vial tops and the injection site

Sharps container

Or a thick plastic bottle with a screw lid

Reconstitution converts the lyophilised powder into the injectable solution. Slow water addition and gentle swirling are the entire technique.

1. R-01Clean both rubber tops (peptide vial and BAC water vial) with an alcohol swab
2. R-02Draw 1–2 ml of BAC water into an insulin syringe
3. R-03Slowly drip the water down the inside wall of the peptide vial. Never spray onto powder
4. R-04Gently swirl the vial for 1–2 minutes until the powder fully dissolves
5. R-05Never shake vigorously: this denatures the peptide
6. R-06Label the vial with the date of reconstitution
7. R-07Store in the refrigerator (2–8°C), good for 4–6 weeks

The arithmetic is the same for every peptide: total mg in the vial divided by ml of BAC water added equals concentration. Then dose ÷ concentration equals draw volume.

Use our retatrutide reconstitution calculator to plug in your vial size, BAC water volume, and target dose. It returns the exact insulin-syringe units to draw.

Subcutaneous means into the fat layer just beneath the skin, not into muscle. Rotate sites daily to avoid lumps and scarring.

Best injection sites · rotate daily

• Belly fat (2 inches from navel)
• Love handles
• Front or outer thigh
• Back of upper arm

Injection technique

1. I-01Clean the skin with an alcohol swab and let it dry
2. I-02Pinch 2–3 inches of fat between your fingers
3. I-03Insert the needle at a 90° angle (straight in)
4. I-04Push the plunger slowly over 3–5 seconds
5. I-05Remove the needle and do not rub the site
6. I-06Dispose of the syringe in a sharps container immediately

Frequency varies by peptide. The GLP-1 class is once weekly because of its long half-life; healing and growth-hormone peptides are typically daily.

Most failures are not exotic. They are one of these six. Read the list once and you will avoid every one.

• Using regular sterile water instead of bacteriostatic water (degrades faster)
• Shaking the vial vigorously (denatures the peptide)
• Injecting into muscle instead of subcutaneous fat
• Not rotating injection sites (causes lumps and scarring)
• Storing reconstituted peptides at room temperature
• Drawing air bubbles into the syringe