How to use peptides
Steps
03
Total Time
< 60 sec
Needle Gauge
31G
Storage
2–8°C
What you will need before opening a vial
Required kit
Five items. All available at PH pharmacies and supply outlets — no prescription required for the consumables.
- Peptide vial
- Lyophilized (freeze-dried) powder
- Bacteriostatic water (BAC water)
- NOT regular water — BAC water contains benzyl alcohol to preserve the peptide
- Insulin syringes
- 0.3 ml or 0.5 ml, 31G needle — available at PH pharmacies
- Alcohol swabs
- For sterilising vial tops and the injection site
- Sharps container
- Or a thick plastic bottle with a screw lid
Step 1 of 3 · ~ 30 seconds
Reconstitute the peptide
Reconstitution converts the lyophilised powder into the injectable solution. Slow water addition and gentle swirling are the entire technique.
- R-01Clean both rubber tops (peptide vial and BAC water vial) with an alcohol swab
- R-02Draw 1–2 ml of BAC water into an insulin syringe
- R-03Slowly drip the water down the inside wall of the peptide vial — never spray onto powder
- R-04Gently swirl the vial for 1–2 minutes until the powder fully dissolves
- R-05Never shake vigorously — this denatures the peptide
- R-06Label the vial with the date of reconstitution
- R-07Store in the refrigerator (2–8°C) — good for 4–6 weeks
Step 2 of 3 · concentration → volume
Calculate & draw your dose
The arithmetic is the same for every peptide: total mg in the vial divided by ml of BAC water added equals concentration. Then dose ÷ concentration equals draw volume.
Worked example
10 mg vial + 2 ml BAC water = 5 mg / ml
For a 500 mcg dose: draw 0.1 ml (10 units) on the insulin syringe
Use our retatrutide reconstitution calculator to plug in your vial size, BAC water volume, and target dose — it returns the exact insulin-syringe units to draw.
Step 3 of 3 · ~ 10 seconds
Inject subcutaneously
Subcutaneous means into the fat layer just beneath the skin — not into muscle. Rotate sites daily to avoid lumps and scarring.
Best injection sites · rotate daily
- Belly fat (2 inches from navel)
- Love handles
- Front or outer thigh
- Back of upper arm
Injection technique
- I-01Clean the skin with an alcohol swab and let it dry
- I-02Pinch 2–3 inches of fat between your fingers
- I-03Insert the needle at a 90° angle (straight in)
- I-04Push the plunger slowly over 3–5 seconds
- I-05Remove the needle and do not rub the site
- I-06Dispose of the syringe in a sharps container immediately
Frequency × time-of-day
Timing by class
Frequency varies by peptide. The GLP-1 class is once weekly because of its long half-life; healing and growth-hormone peptides are typically daily.
| Peptide | Frequency | Best time |
|---|---|---|
| Retatrutide / Tirzepatide / Semaglutide | Weekly | Same day each week |
| BPC-157 | Daily | Morning or night |
| TB-500 | 2× weekly | Any time |
| GHK-Cu | Daily | Morning or night |
| CJC-1295 / Ipamorelin | Daily | Before bed (fasted) |
Six things that will ruin a vial
Common mistakes
Most failures are not exotic — they are one of these six. Read the list once and you will avoid every one.
- Using regular sterile water instead of bacteriostatic water (degrades faster)
- Shaking the vial vigorously (denatures the peptide)
- Injecting into muscle instead of subcutaneous fat
- Not rotating injection sites (causes lumps and scarring)
- Storing reconstituted peptides at room temperature
- Drawing air bubbles into the syringe
Continue the induction
Next steps
Educational reference only. Not medical advice. Confirm peptide-specific dose, frequency, and storage with the manufacturer COA and a licensed clinician before any protocol.